Evercyte

Assay for Testing Nephrotoxicity

Testing of nephrotoxicity of novel drugs using lactate secretion

Besides in vitro assays detecting acute toxicity, especially tests that allow the measurement of chronic toxicity in long-term repeat dosing strategies are of ever increasing importance.
hTERT immortalized human renal cells (RPTEC/TERT1) are treated with the test substances and lactate secretion as a result of cellular toxicity is measured in the supernatant. (Limonciel 2011) 

Testing AON-induced kidney injury

Antisense oligonucleotides (AONs) are getting more and more attention as novel therapies. However, necessary modifications of AONs in order to improve target affinity or stability are associated with safety concerns as AONs induce significant toxicity to kidney proximal tubules.
hTERT immortalized human renal cells (RPTEC/TERT1) are treated with AONs and secretion of EGF as a result of cellular toxicity is measured in the supernatant (Moisan, 2016)

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