Evercyte

HBEC3-KT

hTERT and cdk-4 immortalized human bronchial epithelial cell line
(source: central lung bronchiole)

The cells are characterized by unlimited growth while maintaining expression of cell type specific markers and functions such as:

  • Typical epithelial, cobblestone morphology
  • Expression of the stem cell marker p63
  • Expression of morphological features and markers of clara cells (clara cell specific protein CCSP, surfactant proteins SP-A, SP-D) as well as Type I and II pneumocytes (aquaporin 5, SP-A, SP-C, SP-D) upon 2D differentiation
  • Distinct response to microenvironment

Therefore, these cells are the only solution for gene editing using e.g. the CRISPR/Cas9 system when primary like characteristics are of importance. Additionally, isogenic cell lines that overexpress certain oncogenes or tumor suppressor genes are available as tumor progression model.

For this product you can obtain a license for research use only - read more.

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Database

HBEK3-KT - morphology and expression of funcional markers (download pdf-file)



Publications using HBEC3-KT cell line:

Delgado O, Kaisani AA, Spinola M, Xie XJ, Batten KG, Minna JD, Wright WE, Shay JW. (2011). Multipotent capacity of immortalized human bronchial epithelial cells. PLoS One. 2011;6(7):e22023. doi: 10.1371/journal.pone.0022023. Epub 2011 Jul 7. [PMID: 21760947]

Hu R, Huffman KE, Chu M, Zhang Y, Minna JD, Yu Y. (2016). Quantitative Secretomic Analysis Identifies Extracellular Protein Factors That Modulate the Metastatic Phenotype of Non-Small Cell Lung Cancer. J Proteome Res. 2016 Feb 5;15(2):477-86. [PMID: 26736068]

Levallet G, Vaisse-Lesteven M, Le Stang N, Ilg AG, Brochard P, Astoul P, Pairon JC, Bergot E, Zalcman G, Galateau-Sallé F. (2012). Plasma cell membrane localization of c-MET predicts longer survival in patients with malignant mesothelioma: a series of 157 cases from the MESOPATH Group. J Thorac Oncol. 2012 Mar;7(3):599-606. [PMID: 22246193]

Longhin E, Gualtieri M, Capasso L, Bengalli R, Mollerup S, Holme JA, Øvrevik J, Casadei S, Benedetto CD, Parenti P, Camatini M. (2016) Physico-chemical properties and biological effects of diesel and biomass particles. Environ Pollut. 2016 Aug;215:366-75 [PMID: 27194366]

Ramirez RD, Sheridan S, Girard L, Sato M, Kim Y, Pollack J, Peyton M, Zou Y, Kurie JM, Dimaio JM, Milchgrub S, Smith AL, Souza RF, Gilbey L, Zhang X, Gandia K, Vaughan MB, Wright WE, Gazdar AF, Shay JW, Minna JD.(2004) Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins. Cancer Res. 2004 Dec 15;64(24):9027-34. [PMID: 15604268]

Sato M, Larsen JE, Lee W, Sun H, Shames DS, Dalvi MP, Ramirez RD, Tang H, DiMaio JM, Gao B, Xie Y, Wistuba II, Gazdar AF, Shay JW, Minna JD. (2013). Human lung epithelial cells progressed to malignancy through specific oncogenic manipulations. Mol Cancer Res. 2013 Jun;11(6):638-50. doi: 10.1158/1541-7786.MCR-12-0634-T. Epub 2013 Feb 28. [PMID: 23449933]

Sato M, Vaughan MB, Girard L, Peyton M, Lee W, Shames DS, Ramirez RD, Sunaga N, Gazdar AF, Shay JW, Minna JD. Multiple oncogenic changes (K-RAS(V12), p53 knockdown, mutant EGFRs, p16 bypass, telomerase) are not sufficient to confer a full malignant phenotype on human bronchial epithelial cells. Cancer Res. 2006 Feb 15;66(4):2116-28. [PMID: 16489012]

Vaughan MB, Ramirez RD, Wright WE, Minna JD, Shay JW. (2006). A three-dimensional model of differentiation of immortalized human bronchial epithelial cells.Differentiation. 2006 Apr;74(4):141-8. [PMID: 16683984]

Werner E, Wang H, Doetsch PW. (2015). Role of Pro-inflammatory Cytokines in Radiation-Induced Genomic Instability in Human Bronchial Epithelial Cells. Radiat Res. 2015 Dec;184(6):621-9. doi: 10.1667/RR14045.1. [PMID: 26579942]

Protocols

Please note, Evercyte can only guarantee successful growth of the cells if you stick to the protocols and media provided.

Protocol for in vitro propagation of HBEC3-KT - (download pdf-file)

Protocol for cryoperservation of HBEC3-KT - (download pdf-file)

Documentation

HBEC3-KT cell line

  • is free from human pathogenic viruses HAV, HBV, HCV, HIV, Parvo B19
  • is free from bacterial (incl. mycoplasma) and fungal contaminations
  • is a mixed population of clones with diploid and tetraploid abnormal karyotypes
  • shows telomerase activity
  • is characterized for its STR profile

This material is distributed for research purposes only.

Basic documentation:
Product data sheet (PDS) - download pdf-file 
Certificates of Analysis are available upon request. Please, contact us directly indicating the respective LOT-numbers; mailto: office@evercyte.com 

Legal restriction:
To finalize your order it is required to sign the Material Transfer Agreement (MTA) of Evercyte and an Addendum to this contract as well due to the use of TERT technology.
Download a 
MTA signatory version and the Addendum to be signed by an authorized representative of your institution and submit it to our sales team.