Evercyte

HME1

hTERT immortalized human mammary epithelial cell line 
(source: mammary epithelium)

The cells are characterized by unlimited growth while maintaining expression of cell type specific markers and functions such as:

  • Epithelial morphology and adherent growth
  • Expression of the typical marker protein for mucosal surfaces mucin-1
  • Expression of luminal specific cytokeratins KRT8/18

Therefore, these cells are the only solution for gene editing using e.g. the CRISPR/Cas9 system when primary like characteristics are of importance. Additionally, isogenic cell lines that overexpress certain oncogenes or tumor suppressor genes are available as tumor progression model.

For this product you can obtain a license for research use only - read more.

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Database

HME-1 - morphology and expression of functional markers (download pdf-file)



Publications using HME-1 cell line

Beaudin S, Welsh J. 1,25-Dihydroxyvitamin D induces the glutamate transporter SLC1A1 and alters glutamate handling in non-transformed mammary cells. Mol Cell Endocrinol. 2016 Mar 15;424:34-41. [PMID 26774511]

Beaudin SG, Robilotto S, Welsh J. Comparative regulation of gene expression by 1,25-dihydroxyvitamin D3 in cells derived from normal mammary tissue and breast cancer. J Steroid Biochem Mol Biol. 2015 Apr;148:96-102. Review. [PMID 25239595]

Campo McKnight DA, Sosnoski DM, Koblinski JE, Gay CV. Roles of osteonectin in the migration of breast cancer cells into bone. J Cell Biochem. 2006 Feb 1;97(2):288-302. [PMID 16173048]

Ergun S, Tayeb TS, Arslan A, Temiz E, Arman K, Safdar M, Dağlı H, Korkmaz M, Nacarkahya G, Kırkbeş S, Oztuzcu S. The investigation of miR-221-3p and PAK1 gene expressions in breast cancer cell lines. Gene. 2015 Jan 25;555(2):377-81. [PMID 25447917]

Gogebakan B, Bayraktar R, Suner A, Balakan O, Ulasli M, Izmirli M, Oztuzcu S, Camci C. Do fasudil and Y-27632 affect the level of transient receptor potential (TRP) gene expressions in breast cancer cell lines? Tumour Biol. 2014 Aug;35(8):8033-41. [PMID 24839003]

Gomulkiewicz A, Jablonska K, Pula B, Grzegrzolka J, Borska S, Podhorska-Okolow M, Wojnar A, Rys J, Ambicka A, Ugorski M, Zabel M, Dziegiel P. Expression of metallothionein 3 in ductal breast cancer. Int J Oncol. 2016 Dec;49(6):2487-2497. [PMID 27840910]

Hagemann T, Binder C, Binder L, Pukrop T, Trümper L, Grimshaw MJ. Expression of endothelins and their receptors promotes an invasive phenotype of breast tumor cells but is insufficient to induce invasion in benign cells. DNA Cell Biol. 2005 Nov;24(11):766-76. [PMID 16274297]
Herbert BS, Wright WE, Shay JW. p16(INK4a) inactivation is not required to immortalize human mammary epithelial cells. Oncogene. 2002 Nov 7;21(51):7897-900. [PMID 12420227]

Junk DJ, Vrba L, Watts GS, Oshiro MM, Martinez JD, Futscher BW. Different mutant/wild-type p53 combinations cause a spectrum of increased invasive potential in nonmalignant immortalized human mammary epithelial cells. Neoplasia. 2008 May;10(5):450-61. [PMID 18472962]

Lee H, Park DS, Razani B, Russell RG, Pestell RG, Lisanti MP. Caveolin-1 mutations (P132L and null) and the pathogenesis of breast cancer: caveolin-1 (P132L) behaves in a dominant-negative manner and caveolin-1 (-/-) null mice show mammary epithelial cell hyperplasia. Am J Pathol. 2002 Oct;161(4):1357-69. [PMID 12368209]

Park DS, Lee H, Riedel C, Hulit J, Scherer PE, Pestell RG, Lisanti MP. Prolactin negatively regulates caveolin-1 gene expression in the mammary gland during lactation, via a Ras-dependent mechanism. J Biol Chem. 2001 Dec 21;276(51):48389-97. [PMID 11602600]

Saeed M, Sheff D, Kohen A. Novel positron emission tomography tracer distinguishes normal from cancerous cells. J Biol Chem. 2011 Sep 30;286(39):33872-8. [PMID 21832075]

Tan JM, Chow VT. Cellular expression, localization and interactions of the product of the human MOST-1 gene associated with breast and prostate cancers. Int J Oncol. 2007 Jan;30(1):81-9. [PMID 17143515]

Tzanova T, Gerova M, Petrov O, Karaivanova M, Bagrel D. Synthesis and antioxidant potential of novel synthetic benzophenone analogues. Eur J Med Chem. 2009 Jun;44(6):2724-30. [PMID 18950902]

Zecchin D, Boscaro V, Medico E, Barault L, Martini M, Arena S, Cancelliere C, Bartolini A, Crowley EH, Bardelli A, Gallicchio M, Di Nicolantonio F. BRAF V600E is a determinant of sensitivity to proteasome inhibitors. Mol Cancer Ther. 2013 Dec;12(12):2950-61. [PMID 24107445]

Zhang Y, Chin-Quee K, Riddle RC, Li Z, Zhou Z, Donahue HJ. BRMS1 Sensitizes Breast Cancer Cells to ATP-Induced Growth Suppression. Biores Open Access. 2013 Apr;2(2):77-83. [PMID 23593560]

Protocols

Please note, Evercyte can only guarantee successful growth of the cells if you stick to the protocols and media provided.

Protocol for in vitro propagation of HME1 - (download pdf-file)

Protocol for cryoperservation of HME1 - (download pdf-file)

Documentation

HME1 cell line

  • is free from human pathogenic viruses HAV, HBV, HCV, HIV, Parvo B19
  • is free from bacterial (incl. mycoplasma) and fungal contaminations
  • shows telomerase activity
  • is characterized for its STR profile

This material is distributed for research purposes only.

Basic documentation:
Product data sheet (PDS) - download pdf-file
Certificates of Analysis are available upon request. Please contact us directly indicating the respective LOT-numbers; mailto: office@evercyte.com

Legal restriction:
To finalize your order it is required to sign the Material Transfer Agreement (MTA) of Evercyte and an Addendum to this contract as well due to the use of TERT technology.
Download a 
MTA signatory version and the Addendum to be signed by an authorized representative of your institution and submit it to our sales team.