Evercyte

In Vitro Angiogenesis Models

Angiogenesis describes the formation of the vasculature and is essential throughout normal lifetime as important part in different cellular processes such as tissue regeneration or the pathogenesis of various diseases (e.g. tumor, psoriasis, macular degeneration). As the angiogenic cascade is tightly controlled by a number of pro- or anti-angiogenic factors, the identification and characterization of such factors is an important step in the development of novel therapeutics as well as biosimilars.
Evercyte has established the following assays:

2D proliferation assay for testing the pro- and anti-angiogenic capacity of novel substances

Human hTERT immortalized endothelial cells (HUVEC/TERT2) are seeded into 96-well plates, treated with the compounds of interest, followed by measuring the induction / inhibition of cellular proliferation. Human recombinant VEGF165 has been shown to exhibit high operational stability in HUVEC/TERT2-based proliferation assays. 

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3D spheroid culture for analysis of sprout formation upon treatment with pro- and anti-angiogenic substances

HUVEC/TERT2 are cultivated as 3D spheroids that are then cultivated in the presence of pro- or anti-angiogenic factors. Subsequent sprouting into the surrounding matrix is monitored and gives information on the angiogenic activity of the test substances. Human recombinant VEGF165 has been shown to exhibit high operational stability in HUVEC/TERT2-based sprouting assay. 

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