Evercyte

PODO/TERT256

hTERT immortalized human podocyte cell line
(source: human kidney tissue biopsy)

The cells are characterized by unlimited growth while maintaining expression of cell type specific markers and functions such as:

  • Typical flattened morphology with cytoplasmic protrusions 
  • Appearance of binucleated cells
  • Expression of nephrin, podocin, WT-1, synaptopodin, podocalyxin

Therefore, these cells are the only solution for gene editing using e.g. the CRISPR/Cas9 system when primary like characteristics are of importance. 

For this product you can obtain a license for research use only  - read more

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Availability: In stock

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Database

PODO/TERT256 - morphology and expression of functional markers (download pdf-file)

PODO/TERT256 - growth and morphology (download pdf-file)

PODO/TERT256 - comparison to primary cells (download pdf-file)

Protocols

Please note, Evercyte can only guarantee successful growth of the cells if you stick to the protocols and media provided.

Protocol for in vitro propagation of PODO/TERT256 - (download pdf-file)

Protocol for cryopreservation of PODO/TERT256 - (download pdf-file)

Documentation

PODO/TERT256 cell line

  • is free from human pathogenic viruses HAV, HBV, HCV, HIV, Parvo B19
  • is free from bacterial (incl. mycoplasma) and fungal contaminations
  • shows telomerase activity
  • is characterized for its STR profile

This material is distributed for research purposes only.

Basic documentation:
Product data sheet (PDS) - >download pdf-file
Certificates of Analysis are available upon request. Please, contact us directly indicating the respective LOT-numbers; mailto: office@evercyte.com

Legal restriction:
To finalize your order it is required to sign the Material Transfer Agreement (MTA) of Evercyte and an Addendum to this contract as well due to the use of TERT technology. Download a 
MTA signatory version and the Addendum to be signed by an authorized representative of your institution and submit it to our sales team.