Evercyte

PODO/TERT256

hTERT immortalized human podocyte cell line
(source: human kidney tissue biopsy)

The cells are characterized by unlimited growth while maintaining expression of cell type specific markers and functions such as:

  • Typical flattened morphology with cytoplasmic protrusions 
  • Appearance of binucleated cells
  • Expression of nephrin, podocin, WT-1, synaptopodin, podocalyxin

Therefore, these cells are the only solution for gene editing using e.g. the CRISPR/Cas9 system when primary like characteristics are of importance. 

For this product you can obtain a license for research use only  - read more

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Availability: In stock

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Database

PODO/TERT256 - morphology and expression of functional markers (download pdf-file)

PODO/TERT256 - growth and morphology (download pdf-file)

PODO/TERT256 - comparison to primary cells (download pdf-file)

Protocols

Please note, Evercyte can only guarantee successful growth of the cells if you stick to the protocols and media provided.

Protocol for in vitro propagation of PODO/TERT256 - (download pdf-file)

Protocol for cryopreservation of PODO/TERT256 - (download pdf-file)

Documentation

PODO/TERT256 cell line

  • is free from human pathogenic viruses HAV, HBV, HCV, HIV, Parvo B19
  • is free from bacterial (incl. mycoplasma) and fungal contaminations
  • shows telomerase activity
  • is characterized for its STR profile

This material is distributed for research purposes only.

Basic documentation:
Product data sheet (PDS) - >download pdf-file
Certificates of Analysis are available upon request. Please, contact us directly indicating the respective LOT-numbers; mailto: office@evercyte.com

Legal restriction:
To finalize your order it is required to sign the Material Transfer Agreement (MTA) of Evercyte to this contract.
Download a 
MTA signatory version to be signed by an authorized representative of your institution and submit it to our sales team.