Evercyte

RPTEC/TERT 1

hTERT immortalized human renal proximal tubular epithelial cell line

The cells are characterized by unlimited growth while maintaining expression of cell type specific markers and functions such as:

  • Typical epithelial, cobblestone morphology
  • Functional tight junctions as evidenced by ZO-1 expression, dome formation and trans epithelial electrical resistance (TEER) levels like normal cells
  • Gamma glutamyl-transferase activity
  • Expression of Aminopetidase-N and E-cadherin
  • Response to parathyroid hormone (PHT) treatment by increased cAMP production
  • Transporter activity when differentiated (MRP2, MRP4, OAT4, MDR1, MATE1, OCTN2, OCT3)

Therefore, these cells are the only solution for gene editing using e.g. the CRISPR/Cas9 system when primary like characteristics are of importance.

Furthermore, in collaboration with The Human Protein Atlas next generation sequencing was performed and data are available for download to support your decision process in selecting cells that are fit for purpose - get these data after log in from our database below.

For this product you can obtain a license for research use only - read more.

Contact us for more information

Availability: In stock

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Database

NGS data for download (download xlsx-file)

RPTEC/TERT1 - morphology and marker expression (download pdf-file)



Publications using RPTEC/TERT1 cells
 

Moisan A, et al. Inhibition of EGF Uptake by Nephrotoxic Antisense Drugs In Vitro and Implications for Preclinical Safety Profiling. Molecular Therapy: Nucleic Acids. 2017; 6: 89-105. [PMID 28325303]

Wilmes A, et al. Mechanism of cisplatin proximal tubule toxicityrevealed by integrating transcriptomics, proteomics, metabolomics and biokinetics. Toxicol In Vitro. 2015 Dec 25;30(1 Pt A):117-27. [PMID 25450742]

Aschauer L, et al. Expression of xenobiotic transporters in the human renal proximal tubule cell line RPTEC/TERT1. Toxicol In Vitro. 2015 Dec 10. pii: S0887-2333(14)00243-4. [PMID 25500123]

Aschauer L, et al. Application of RPTEC/TERT1 cells for investigation of repeat dose nephrotoxicity: A transcriptomic study. Toxicol In Vitro. 2015 Dec 25;30(1 Pt A):106-16. [PMID 25450743]

Kramer N.I., et al. Biokinetics in repeated-dosing in vitro drug toxicity studies. Toxicol In Vitro. 2015 Sep 8. pii: S0887-2333(15)00218-0. [PMID 26362508]

Slyne J, et al., New developments concerning the proximal tubule in diabetic nephropathy: in vitro models and mechanisms. Nephrol Dial Transplant. 2015 Aug;30.
[PMID 26209740]

Simon-Friedt BR, et al. The RPTEC/TERT1 Cell Line as an Improved Tool for In Vitro Nephrotoxicity Assessments. Biol Trace Elem Res. 2015 Jul;166(1):66-71. [PMID 25893367]

Ranninger C., et al. Nephron Toxicity Profiling via Untargeted Metabolome Analysis Employing a High Performance Liquid Chromatography-Mass Spectrometry-based Experimental and Computational Pipeline. J Biol Chem. 2015 Jul 31;290(31):19121-32. [PMID 26055719]

Maschmeyer I, et al. A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents. Lab Chip. 2015 Jun 21;15(12):2688-99. [PMID 25996126]

Fliedl L, et al. Optimisation of a quantitative PCR based method for Plasmid Copy Number Determination in Human Cell Lines. N Biotechnol. 2015 Mar 18. pii: S1871-6784(15)00046-1. [PMID 25796475]

Limonciel A., et al. Transcriptomics hit the target: Monitoring of ligand-activated and stress response pathways for chemical testing. Toxicol In Vitro. 2015 Jan 13. pii: S0887-2333(14)00251-3. [PMID 25596134]

Jennings P, Crean D, Aschauer L, Limonciel A, Moenks K, Kern G, Hewitt P, Lhotta K, Lukas A, Wilmes A, Leonard MO. Interleukin-19 as a translational indicator of renal injury. Arch Toxicol. 2015 Jan;89(1):101-6. [PMID 24714768]

Crean D., et al. Development of an in vitro renal epithelial disease state model for xenobiotic toxicity testing. Toxicol In Vitro. 2014 Dec 20. pii: S0887-2333(14)00239-2. [PMID 25536518]

Wilmes A, et al.  Evidence for a role of claudin 2 as a proximal tubular stress responsive paracellular water channel. Toxicol Appl Pharmacol. 2014 Sep 1;279(2):163-72. [PMID 24907557]

Fliedl L, Wieser M, Manhart G, Gerstl MP, Khan A, Grillari J, Grillari-Voglauer R. Controversial Role of Gamma-Glutamyl Transferase Activity in Cisplatin Nephrotoxicity. ALTEX. 2014;31(3):269-78. [PMID 24664430]

Simon BR, et al. Cadmium alters the formation of benzo[a]pyrene DNA adducts in the RPTEC/TERT1 human renal proximal tubule epithelial cell line. Toxicol Rep. 2014 Jul 14;1:391-400. [PMID 25170436]

Aschauer L, et al. Delineation of the Key Aspects in the Regulation of Epithelial Monolayer Formation. Mol Cell Biol. 2013 Jul;33(13):2535-50. [PMID 23608536]

Wilmes A, et al. Application of integrated transcriptomic, proteomic and metabolomic profiling for the delineation of mechanisms of drug induced cell stress. J Proteomics. 2013 Feb 21;79:180-94. [PMID 23238060]

Jennings P, et al. Transcriptomic alterations induced by Ochratoxin A in rat and human renal proximal tubular in vitro models and comparison to a rat in vivo model. Arch Toxicol. 2012 Apr;86(4):571-89.  [PMID 22124623]

Limonciel A, et al. Oxidative stress induced by potassium bromate exposure results in altered tight junction protein expression in renal proximal tubule cells. Arch Toxicol. 2012 Nov;86(11):1741-51. [PMID 22760423]

Radford R, et al. Carcinogens induce loss of the primary cilium in human renal proximal tubular epithelial cells independently of effects on the cell cycle. Am J Physiol Renal Physiol. 2012 Apr 15;302(8):F905-16. doi: 10.1152/ajprenal.00427.2011. Epub 2012 Jan 18. [PMID 22262483]
Erratum in: Am J Physiol Renal Physiol. 2013 Sep 1;305(5):F796. Blaque, Oliver [corrected to Blacque, Oliver]. [PMID 22262483]

Limonciel A, et al. Lactate is an ideal non-invasive marker for evaluating temporal alterations in cell stress and toxicity in repeat dose testing regimes. Toxicol In Vitro. 2011 Dec;25(8):1855-62. [PMID 21635945]

Sarkozi R. et al. Oncostatin M is a novel inhibitor of TGF-1-induced matricellular protein expression. Am J Physiol Renal Physiol 2011 Nov, 301(5):F1014-F1025.
[PMID 21816755]

Ellis JK, et al. Metabolic response to low-level toxicant exposure in a novel renal tubule epithelial cell system. Mol Biosyst. 2011 Jan;7(1):247-57.  [PMID 21103459]

Wieser M, et al. hTERT alone immortalizes epithelial cells of renal proximal tubules without changing their functional characteristics. Am J Physiol Renal Physiol. 2008 Nov;295(5):F1365-75. [PMID 18715936]


 

Protocols

Please note, Evercyte can only guarantee successful growth of the cells if you stick to the protocols and media provided. 

Protocol for in vitro propagation - (download pdf-file)

Protocol for cryopreservation - (download pdf-file)

Documentation

RPTEC/TERT1 cell line

  • is free from human pathogenic viruses HAV, HBV, HCV, HIV, Parvo B19
  • is free from bacterial (incl. mycoplasma) and fungal contaminations
  • does not produce retroviral particles
  • shows a normal diploid karyotype
  • shows telomerase activity
  • is characterized for its STR profile

This material is distributed for research purposes only.

Basic documentation:
Product data sheet (PDS) - download pdf-file
Certificates of Analysis are available upon request. Please, contact us directly indicating the respective LOT-numbers; mailto: office@evercyte.com

Legal restriction:
To finalize your order it is required to sign the Material Transfer Agreement (MTA) of Evercyte and an Addendum to this contract as well due to the use of TERT technology.
Download a 
MTA signatory version and the Addendum to be signed by an authorized representative of your institution and submit it to our sales team.