Evercyte

Vision & Strategy

Our vision is to provide relevant human cell lines as model systems for cell based assays, production host, as well as for cellular therapy applications.

For cell based assays we provide relevant and standardised immortalized human cell lines that retain cell type specific functionalities but still divide continuously as well as cell based assays based on our cell lines (e.g. for REACH). Our cell lines will lead to replacement, reduction, and refinement (‘3R’s) of animal trials as well as of cells that do not reflect the tissue specific physiology of a cell based assay in question.

For production host cell lines, we provide human cells as novel host cell lines for production of biopharmaceuticals including recombinant proteins or vaccines, whenever a human glycosylation or other post-translational modification is necessary.

For the use in cell based therapies, especially the induced pluripotent cells (iPSCs) non-invasively isolated from human urine and non-integratively reprogrammed are considered a valuable source for therapeutic applications. Similarly, mesenchymal stem cells (MSCs) can be isolated from human urine for autologous therapeutic strategies.

 

Strategic Technology

We apply two main technologies for generation of human cell lines that maintain their primary like functionality.

The first technology for generation of human cell lines is by immortalization using the catalytic subunit of human telomerase (hTERT). Using ectopic overexpression of hTERT, cells can be immortalized without losing key cell type specific functionalities. For this, we have licenced in hTERT based patents from Geron Corp.

The second technology is based on reprogramming human urine derived cells. This technology has been developed in cooperation with Guangzhou Institute of Biomedicine and health (GIBH), Chinese Academy of Sciences, China and is refined using integration free repromming based on mRNA transfections, licenced in from Fraunhofer Gesellschaft.


hTERT immortalized cells

Normal human somatic cells can only be propagated for a limited number of population doublings before entering a phase of irreversible growth arrest also termed replicative senescence. This phase is mainly due to progressive telomere erosion with each cell division. Since replicative senescence together with the restricted availability of specialized human normal cells limits the use of in vitro grown cells cells as in vitro test systems. Therefore, senescence has to be circumvented, which can be achieved by several strategies.

Evercyte makes mainly use of the technology of reactivation of human telomerase by overexpression of the catalytic subunit of this enzyme (hTERT). Additionally, if needed, overexpression of hTERT is combined with ectopic expression of viral oncogenes such as SV40 large T and small t antigens.

Thereby, we succeed in providing sufficient amounts of special human cell lines with characteristics of the normal counterpart cells that are found in vivo.

Besides the isolation, immortalization and characterization of human somatic cells, we also have developed a method for the generation of induced pluripotent stem cells from any consenting individual in a non-invasive form. Renal epithelial cells exfoliated into the urine can be reprogrammed into pluripotent stem cells by non-viral techniques. This method is advantageous in many circumstances, as the isolation of urinary cells is simple, cost-effective and universal.

more about our hTERT immortalized cells