hTERT and cdk4 immortalized human colon epithelial cell line

The cells are characterized by unlimited growth while maintaining expression of cell type specific markers and functions such as:

  • Typical epithelial morphology when growth arrested 
  • Expression of A33, mucin 1 and villin in differentiated cells 
  • Formation of cyst-like structures upon 3D differentiation

Therefore, these cells are the only solution for gene editing using e.g. the CRISPR/Cas9 system when primary like characteristics are of importance. Additionally, isogenic cell lines that overexpress certain oncogenes or tumor suppressor genes are available as tumor progression model. 

For this product you can obtain a license for research use only - read more.

Contact us for more information.


HCEC-1CT - morphology and expression of functional markers (download pdf-file)

HCEC-1CT - 3D differentiation (download pdf-file)

Publications using HCEC-1CT:

Eskiocak U, Kim SB, Ly P, Roig AI, Biglione S, Komurov K, Cornelius C, Wright WE, White MA, Shay JW. (2011) Functional parsing of driver mutations in the colorectal cancer genome reveals numerous suppressors of anchorage-independent growth. Cancer Res. 2011 Jul 1;71(13):4359-65. [PMID: 21527559]

Khare V, Dammann K, Asboth M, Krnjic A, Jambrich M, Gasche C. (2015) Overexpression of PAK1 promotes cell survival in inflammatory bowel diseases and colitis-associated cancer. Inflamm Bowel Dis. 2015 Feb;21(2):287-96. [PMID: 25569743]

Roig AI, Eskiocak U, Hight SK, Kim SB, Delgado O, Souza RF, Spechler SJ, Wright WE, Shay JW. (2010)Immortalized epithelial cells derived from human colon biopsies express stem cell markers and differentiate in vitro. Gastroenterology. 2010 Mar;138(3):1012-21.e1-5. [PMID: 19962984]

Vejdovszky K, Sack M, Jarolim K, Aichinger G, Somoza MM, Marko D. (2017) In vitro combinatory effects of the Alternaria mycotoxins alternariol and altertoxin II and potentially involved miRNAs. Toxicol Lett. 2017 Feb 5;267:45-52. [PMID: 28007639]

Warth B, Del Favero G, Wiesenberger G, Puntscher H, Woelflingseder L, Fruhmann P, Sarkanj B, Krska R, Schuhmacher R, Adam G, Marko D. (2016) Identification of a novel human deoxynivalenol metabolite enhancing proliferation of intestinal and urinary bladder cells. Sci Rep. 2016 Sep 23;6:33854. [PMID: 27659167]


Please note, Evercyte can only guarantee successful growth of the cells if you stick to the protocols and media provided. 

Protocol for in vitro propagation of HCEC-1CT - (download pdf-file)

Protocol for cryoperservation of HCEC-1CT - (download pdf-file)


HCEC-1CT cell line

  • is free from human pathogenic viruses HAV, HBV, HCV, HIV, Parvo B19
  • is free from bacterial (incl. mycoplasma) and fungal contaminations
  • shows telomerase activity
  • is characterized for its STR profile

This material is distributed for research purposes only

Basic documentation:
Product data sheet (PDS) - download pdf-file
Certificates of Analysis are available upon request. Please, contact us directly indicating the respective LOT-numbers; mailto: office@evercyte.com

Legal restriction:
To finalize your order it is required to sign a Material Transfer Agreement (MTA) of Evercyte.
Download a 
MTA signatory version to be signed by an authorized representative of your institution and submit it to our sales team (office@evercyte.com).