hTERT and SV40 early region immortalized human dermal keratinocyte cell line

The cells are characterized by unlimited growth while maintaining expression of cell type specific markers and functions such as:

  • Typical epithelial, cobblestone appearance
  • Differentiation and stratification in a 3D skin equivalent 
  • Expression of keratin 1, keratin 2, keratin 10 and loricrin after 3D differentiation

Therefore, these cells are the only solution for gene editing using e.g. the CRISPR/Cas9 system when primary like characteristics are of importance.

For this product you can obtain a license for research use only - read more.

Contact us for more information


NHEK/SVTERT3-5 - morphology and expression of functional markers (download pdf-file)


Please note, Evercyte can only guarantee successful growth of the cells if you stick to the protocols and media provided.

Protocol for in vitro propagation of NHEK/SVTERT3-5 - (download pdf-file)

Protocol for cryopreservation of NHEK/SVTERT3-5 - (download pdf-file)


NHEK/SVTERT3-5 cell line

  • is free from human pathogenic viruses HAV, HBV, HCV, HIV, Parvo B19
  • is free from bacterial (incl. mycoplasma) and fungal contaminations
  • shows telomerase activity
  • is characterized for its STR profile

This material is distributed for research purposes only.

Basic documentation:
Product data sheet (PDS) - download pdf-file
Certificates of Analysis are available upon request. Please, contact us directly indicating the respective LOT-numbers, mailto: office@evercyte.com

Legal restriction:
To finalize your order it is required to sign a Material Transfer Agreement (MTA) of Evercyte.
Download a 
MTA signatory version to be signed by an authorized representative of your institution and submit it to our sales team (office@evercyte.com).