TGFß Induced Fibrosis Model

Transforming growth factor ß (TGFß) plays an essential role in fibrotic reactions and thus contributes significantly to various disorders of connective tissues such as diabetic nephropathy, rheumatoid arthritis, radiation-induced fibrosis, myocarditis or problems with wound healing and scar formation.
Evercyte offers cells and assays for the identification of potential activators or inhibitors of TGFß induced signaling.

Assay for testing substances targeting TGFß as essential mediator of fibrosis.

Treatment of human hTERT immortalized fibroblasts (fHDF/TERT166) with TGFß induces a concentration dependent increase in α-smooth muscle actin expression. The cellular response is measured using a novel immunofluorescence protocol that allows measurement in 96- well plate format.

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